Concerning potency analysis, while several published methods exist, the available methods have not been validated to the level of a standard method. The following is a list of appropriate reference methods. Potency methods must be validated extensively to ensure they meet the requirements of testing.
Methods applicable to potency determination:
- Association of Analytical Communities (AOAC) 2018. “Cannabinoid in Dried Flowers and Oil, 2018.10”
- Association of Analytical Communities (AOAC) 2018. “Quantitation of Cannabinoids in Cannabis Dried Plant Materials, Concentrates, and Oils, 2018.11 ”
- Backer, Benjamin De., et al., 2009. Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material.Journal of Chromatography B, 887 4115-4124.
- Gambaro, Veniero., et al., 2002. Determination of primary active constituents in Cannabis preparations by high-resolution gas chromatography/flame ionization detection and high-performance liquid chromatography/UV detection. Analytica Chimica Acta 468, 245-254.
- L. Ambach, F. Penitschka, A. Broillet, S. König, W. Weinmann., 2014. Simultaneous quantification of delta-9-THC, THC-acid A, CBN and CBD in seized drugs using HPLC-DAD. Forensic Science International 243, 107-111.
- Stolker, A.A.M., et al., 2004. Determination of cannabinoids in cannabis products using liquid chromatography-ion trap mass spectrometry. Journal of Chromatography A, 1058, 143-151.
- Swift, Wendy., et al., 2013. Analysis of Cannabis Seizures in NSW, Australia: Cannabis Potency and Cannabinoid Profile, PLOS One v.8 i.7 e70052.
- Upton, Roy., et al., 2014. Cannabis Inflorescence Cannabis Spp.: Standards of Identity, Analysis, And Quality Control. American Herbal Pharmacopoeia.
- Elhendawy, Mostafa A., Radwan, Mohamed M., Ibrahim, Elsayed A., Wanas, Amira S., Chandra, Suman., Godfrey, Murrell., ElSohly, Mahmoud. 2024. Validation and Quantitation of Fifteen Cannabinoids in Cannabis and Marketed Products Using High-Performance Liquid Chromatography-Ultraviolet/Photodiode Array Method. Cannabis and Cannabinoid Research, 9:4, 1091-1107.
- Marios C. Christodoulou, Atalanti Christou, Ioannis J. Stavrou, Constantina P. Kapnissi-Christodoulou. 2019. Evaluation of different extraction procedures for the quantification of seven cannabinoids in cannabis-based edibles by the use of LC-MS. Journal of Food Composition and Analysis, 115.